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Autor/inn/en | Werner, R. Marshall; Johnson, Austin |
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Titel | [superscript 31]P NMR of the Pyruvate Kinase Reaction: An Undergraduate Experiment in Enzyme Kinetics |
Quelle | In: Biochemistry and Molecular Biology Education, 45 (2017) 6, S.509-514 (6 Seiten)Infoseite zur Zeitschrift
PDF als Volltext |
Zusatzinformation | ORCID (Werner, R. Marshall) |
Sprache | englisch |
Dokumenttyp | gedruckt; online; Zeitschriftenaufsatz |
ISSN | 1470-8175 |
DOI | 10.1002/bmb.21079 |
Schlagwörter | Undergraduate Students; Science Instruction; Biochemistry; Spectroscopy; Science Experiments; Teaching Methods |
Abstract | Understanding how to perform an enzyme assay is a critical learning skill in the undergraduate biochemistry curriculum. Students in biochemistry typically have been exposed to the use of NMR spectroscopy as a tool to determine chemical structure, but rarely are they exposed to the utility of NMR to evaluate enzyme kinetics. Furthermore, coverage of NMR experiments utilizing "alternative nuclei," such as [superscript 15]N, [superscript 19]F, and [superscript 31]P may be neglected. Herein we report a simple 31P NMR tube experiment that allows students to examine the enzyme kinetics and equilibrium constant of the reaction catalyzed by pyruvate kinase. (As Provided). |
Anmerkungen | Wiley-Blackwell. 350 Main Street, Malden, MA 02148. Tel: 800-835-6770; Tel: 781-388-8598; Fax: 781-388-8232; e-mail: cs-journals@wiley.com; Web site: http://www.wiley.com/WileyCDA |
Erfasst von | ERIC (Education Resources Information Center), Washington, DC |
Update | 2020/1/01 |