Effects of Post-Training Hippocampal Injections of Midazolam on Fear Conditioning

Autor/inn/en	Gafford, Georgette M.; Parsons, Ryan G.; Helmstetter, Fred J.
Titel	Effects of Post-Training Hippocampal Injections of Midazolam on Fear Conditioning
Quelle	In: Learning & Memory, 12 (2005) 6, S.573-578 (6 Seiten) PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1072-0502
Schlagwörter	Animals; Drug Use; Learning Processes; Brain Hemisphere Functions; Memory; Fear; Classical Conditioning + Suchen Sie Ihr Suchwort? Animal; Tier; Tiere; Drug consumption; Substance abuse; Drogenkonsum; Learning process; Lernprozess; Gedächtnis; Furcht
Abstract	Benzodiazepines have been useful tools for investigating mechanisms underlying learning and memory. The present set of experiments investigates the role of hippocampal GABA[subscript A]/benzodiazepine receptors in memory consolidation using Pavlovian fear conditioning. Rats were prepared with cannulae aimed at the dorsal hippocampus and trained with a series of white noise--shock pairings. In the first experiment, animals received intrahippocampal infusion of midazolam or vehicle immediately or 3 h after training. Then, 24 h later, freezing to the training context and the white noise were measured independently. Results show infusion of midazolam immediately, but not 3 h, after training selectively attenuates contextual fear conditioning. In the second experiment, animals received intrahippocampal infusions of an antisense oligodeoxynucleotide (ODN) targeting the [alpha]5 subunit of the GABA[subscript A] receptor or a missense control for several days prior to training and testing. Immediately after training, animals received an infusion of either midazolam or vehicle. Western blots conducted after testing showed a significant decrease in [alpha]5-containing GABA[subscript A] receptor protein. This reduction did not alter the effectiveness of midazolam immediately after training at impairing context fear memory. Therefore, [alpha]5-containing GABA[subscript A] receptors may not contribute to the effects of midazolam on context fear conditioning when given immediately post-training. (Contains 3 figures.) (Author).
Anmerkungen	Cold Spring Harbor Laboratory Press. 500 Sunnyside Boulevard, Woodbury, NY 11797-2924. Tel: 800-843-4388; 516-367-8800; Fax: 516-422-4097; e-mail: cshpres@cshl.edu; Web site: http://www.learnmem.org/
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10