Improvements in Skills and Knowledge after a Comprehensive ELISA Teaching Course for Biotechnology Undergraduates

Autor/inn/en	Yan, Jingmin; Chen, Jiangao; Mao, Xuhu; Li, Qian
Titel	Improvements in Skills and Knowledge after a Comprehensive ELISA Teaching Course for Biotechnology Undergraduates
Quelle	In: Biochemistry and Molecular Biology Education, 51 (2023) 4, S.418-427 (10 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Yan, Jingmin)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21739
Schlagwörter	Undergraduate Students; Science Instruction; Biotechnology; Biochemistry; College Science; Diagnostic Tests; Genetics; Laboratory Experiments; Science Process Skills; Molecular Biology; Communicable Diseases + Suchen Sie Ihr Suchwort? Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biotechnologie; Biochemie; Diagnostic test; Diagnostischer Test; Humangenetik; Laboratory work; Laborarbeit; Molekularbiologie; Contagious disease; Contagious diseases; Communicable disease; Infektionskrankheit
Abstract	As a universal and extensively adopted technique, enzyme-linked immunosorbent assay (ELISA) can be used to detect and quantify small molecules in many applications both clinical and analytical. However, generally, students experiment mechanically using commercial ELISA kits according to the instructions and eventually produce a standard curve to calculate the concentration of the sample to be measured, cannot understand the critical factors and process of method establishment. This study systematically introduced undergraduates to using the pathogen-specific antigen and establishing an indirect ELISA method to detect the diagnostic target pathogen "Burkholderia pseudomallei." This course aimed to develop the experimental skills of the students and improve their scientific research knowledge, which fully embody the organic combination of scientific research and teaching. Students independently selected the diagnostic antigen target of interest, obtained the antigen proteins using genetic engineering techniques, and established an ELISA method through a series of conditional optimization experiments. In addition, typical student-generated data, experimental methods, and a student feedback interpretation are presented in this study. Overall, the students were able to combine abstract knowledge with practice and understand the principles and applications of antigen--antibody interactions, thus enabling them to gain practical experience in molecular biology techniques, and learn how to use this principle to establish an ELISA method for detecting infectious diseases. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01