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| Autor/inn/en | Marquioni, Vinícius; Nunes, Francis Morais Franco; Novo-Mansur, Maria Teresa Marques |
|---|---|
| Titel | Protein Identification by Database Searching of Mass Spectrometry Data in the Teaching of Proteomics |
| Quelle | In: Journal of Chemical Education, 98 (2021) 3, S.812-823 (12 Seiten)Infoseite zur Zeitschrift
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| Zusatzinformation | ORCID (Nunes, Francis Morais Franco) ORCID (Novo-Mansur, Maria Teresa Marques) |
| Sprache | englisch |
| Dokumenttyp | gedruckt; online; Zeitschriftenaufsatz |
| ISSN | 0021-9584 |
| Schlagwörter | Spectroscopy; Biochemistry; Databases; Science Instruction; Undergraduate Students; Search Engines; Online Searching; Data Analysis |
| Abstract | Mass spectrometry is essential for large-scale protein identification in proteomics. By database searching of mass spectrometry data, it is possible to identify proteins without the need to interpret MS/MS spectra (de novo sequencing), which is time-consuming and not feasible as a systematic tool in proteomic analysis. Many authors have reported initiatives to teach protein mass spectrometry to undergraduate students, but database searching is usually not emphasized. Here, we propose a class about database searching of mass spectrometry data for protein identification in order to complement a theoretical proteomics course. It includes a presentation about the theory of database searching and a practical, computer-based activity in which students can learn how to perform analysis of mass spectrometry data for proteomics purposes. Our class model fills a widespread gap in the teaching of proteomics and improves students' understanding of protein identification by mass spectrometry. (As Provided). |
| Anmerkungen | Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
| Erfasst von | ERIC (Education Resources Information Center), Washington, DC |
| Update | 2024/1/01 |
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