Measurement of Enzyme Kinetics by Use of a Blood Glucometer: Hydrolysis of Sucrose and Lactose

Autor/inn/en	Heinzerling, Peter; Schrader, Frank; Schanze, Sascha
Titel	Measurement of Enzyme Kinetics by Use of a Blood Glucometer: Hydrolysis of Sucrose and Lactose
Quelle	In: Journal of Chemical Education, 89 (2012) 12, S.1582-1586 (5 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/ed200735f
Schlagwörter	Teaching Methods; Biochemistry; Kinetics; Science Experiments; Science Laboratories; Diabetes; College Science; Science Instruction; Food; Scientific Concepts + Suchen Sie Ihr Suchwort? Teaching method; Lehrmethode; Unterrichtsmethode; Biochemie; Kinetik; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Lebensmittel
Abstract	An alternative analytical method for measuring the kinetic parameters of the enzymes invertase and lactase is described. Invertase hydrolyzes sucrose to glucose and fructose and lactase hydrolyzes lactose to glucose and galactose. In most enzyme kinetics studies, photometric methods or test strips are used to quantify the derivates of the substrates. The use of a commercial blood glucose meter to determine the hydrolyzed glucose is described. This inexpensive and efficient method can be used when teaching enzymatic kinetics in lower-level biochemistry laboratories. (Contains 11 figures.) (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10