An Undergraduate Investigation into the 10-23 DNA Enzyme that Cleaves RNA: DNA Can Cut It in the Biochemistry Laboratory

Autor/inn/en	Flynn-Charlebois, Amber; Burns, Jamie; Chapelliquen, Stephanie; Sanmartino, Holly
Titel	An Undergraduate Investigation into the 10-23 DNA Enzyme that Cleaves RNA: DNA Can Cut It in the Biochemistry Laboratory
Quelle	In: Journal of Chemical Education, 88 (2011) 2, S.226-228 (3 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9584
DOI	10.1021/ed100607k
Schlagwörter	Undergraduate Students; Genetics; Science Instruction; Science Laboratories; Biotechnology; Biochemistry; College Science; Hands on Science; Kinetics; Scientific Concepts + Suchen Sie Ihr Suchwort? Humangenetik; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biotechnologie; Biochemie; Kinetik
Abstract	A low-cost biochemistry experiment is described that demonstrates current techniques in the use of catalytic DNA molecules and introduces a nonradioactive, nonfluorescent, inexpensive, fast, and safe method for monitoring these nucleic acid reactions. The laboratory involves the exploration of the 10-23 DNA enzyme as it cleaves a specific RNA substrate, and the reaction is visualized qualitatively following gel electrophoresis. This experiment provides the students with hands-on experience manipulating nucleic acids using polyacrylamide gel electrophoresis to visually follow the kinetics of the reaction and introduces the student to a novel postelectrophoretic stain, cresyl violet, for detection of the nucleic acids. These DNA enzyme catalysts have potential applications in both biotechnology and medicine and therefore should be of great interest to upper-level undergraduate students. (Contains 4 figures.) (As Provided).
Anmerkungen	Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10